Comparison of Assemblies from Reads of Different Sequencing Technologies.

1- for Sanger reads there were no contigs assembled from combining more than one read, therefore the term contigs represents Sanger reads that were longer than 500 bp. 2– as there were no contigs assembled from more than one read, then there cannot be any ‘chimeric’ contigs. This table compares assembly statistics for assemblies of reads from quality-trimmed Illumina reads, Sanger reads, and pyrosequencing reads (quality trimming was performed by assembly software). The upper portion of the table compares different statistics related to contig lengths, and the lower portion compares statistics related to the accuracy of the contigs. N50 is defined as the length N for which 50% of all bases are represented in fragments of length L