Data from: Modified low-salt CTAB extraction of high-quality DNA from contaminant rich tissues

The increasing use of high-throughput sequencing platforms has made the
isolation of pure, high molecular weight DNA a primary concern for studies
of a diverse range of organisms. Purification of DNA remains a significant
challenge in many tissue and sample types due to various organic and
inorganic molecules that co-precipitate with nucleic acids. Molluscs, for
example, contain high concentrations of polysaccharides which often
co-precipitate with DNA and can inhibit downstream enzymatic reactions. We
modified a low-salt CTAB (MoLSC) extraction protocol to accommodate
contaminant rich animal tissues and compared this method to a standard
CTAB extraction protocol and two commercially available animal tissue DNA
extraction kits using oyster adductor muscle. Comparisons of purity and
molecular integrity showed that our in-house protocol yielded genomic DNA
generally free of contaminants and shearing, whereas the traditional CTAB
method and some of the commercial kits yielded DNA unsuitable for some
applications of massively parallel sequencing (MPS). Our open-source MoLSC
protocol provides a cost-effective, scalable, alternative DNA extraction
method that can be easily optimized and adapted for sequencing
applications in other contaminant rich samples.