Mutant strain survival frequencies.

SD, standard deviation; NHEJ, non-homologous end joining. aBoth YDV1.17 and YDV1.12 are compared to their mutant derivative strains, and not to each other. Thus, for both strains, the fold change is set to 1.00 and the p-value is not calculated, as indicated by “–” in the table. bFold change is calculated as the Mean mutant/Mean wild type. cP-values are calculated using a two-tailed paired t-test by comparing mutant strains to the wild type parental strain. dNo hygromycin resistant colonies were detected in the triplicate experiments testing these mutants. Therefore, the hygromycin resistant survival was calculated as less than the average of 1 survivor in each of these experiments.