HLA coverage per cell line.

The distribution of unique self-peptides eluted from uninfected cells are shown for BLCL 1053 (A), 1077 (B), 1090 (C) and 1112 (D). Each slice of the pie represents the fraction of unique peptides eluted from that specific BLCL presented by the specific HLA molecule. Peptides that could not be reliably assigned to any of the HLA molecules expressed are depicted as None. The Venn diagram in (E) reveals the overlap in HLA-A*02:01 binding peptides eluted from the three HLA-A*02:01-expressing BLCL. In (F) the copy number of eluted HLA-A*02:01 peptides is shown for the three HLA-A*02:01-expressing BLCL. The copy number of peptides eluted from only 1 BLCL is significantly lower compared to peptides eluted from ≥2 BLCL (p<0.05, Kruskall Wallis test).