Gene expression profile at single cell level of CD45+ cells from AB1 mesothelioma treated with either vaccinia virus Tiantan strain or mock-treated

To understand the immune infiltrates in the tumor microenvironment in response to vaccinia virotherapy, we used single cell RNA sequencing (scRNAseq) to analyze the diversity of total CD45+ isolated from AB1 mesothelioma Overall design: Balb/c mice bearing 7-day-old subcutaneous AB1 tumors were intratumorally treated with either modified vaccinia virus Tiantan strain (MVTT) or mock-treated, total tumor-infiltrating CD45+ cells were sorted by flow cytometry 2 days post treatment and processed in the BD Rhapsody system for scRNA-seq