Loss of p53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells

To understand the molecular and cellular impact of p53 loss on gene expression profile of multiple myeloma cells, we generated isogenic pairs of TP53 wild-type and knockout cells by CRISPR/Cas9. We then performed RNAseq analysis using data obtained from RNA-seq of three p53-KO clones and one scrambled control cells. Comparative gene expression profiling analysis of RNA-seq data for MM.1S CRISPR p53-KO cells (A7, B6, B16 clones) and its non-targeted scrambled control (Scr) cells.