Source images of the Eocene amber fossil Histiogaster mites, illustrating the resolution of confocal microscopy

Source images for the paper Beyond the limits of light: an application of Super-Resolution Confocal Microscopy (cCLSM) to investigate Eocene amber microfossils by Dmitry D. Vorontsov, Vasiliy Kolesnikov, Elena E. Voronezhskaya, Evgeny Perkovsky, Marielle M. Berto, Joseph Mowery, Ronald Ochoa and Pavel B. Klimov <br> Confocal scanning microscopy was done using a Zeiss LSM-880 CLSM equipped with an Airyscan super-resolution detector. We used a 488 nm Argon laser for excitation, while the detection range was set from ca. 500 to 700 nm in traditional CLSM mode, while in sCLSM mode (Airyscan detector) a set of emission filters, BP 495–550 and LP 570, was used. These settings allowed to detect green to yellow and red fluorescence. In Airyscan mode, a 63×, n.a. 1.45 objective was used.