Euphausiid-specific metabarcoding data from eDNA samples and video footage collected on the TEMPO voyage

This dataset was collected on the TEMPO voyage in 2021 in the Southern Ocean of East Antarctica. The data contains A) genetic data from eDNA samples and B) video footage from a CTD-mounted camera. The eDNA samples were collected by filtering five litres of seawater in the East Antarctic Southern Ocean from the surface and seafloor from CTD samples as well as from the uncontaminated seawater line in and near krill swarms(total n = 110). After DNA extraction, a Euphausiid-specific metabarcoding marker was used to determine the presence of different Southern Ocean krill species in each sample. The CTD-mounted camera collected ~5 min of video footage at the surface and seafloor at each CTD station. Detailed methods are available in the following publication: Suter L et al. (2025) Monitoring Antarctic krill (Euphausia superba) distribution in the Southern Ocean: environmental DNA (eDNA) adds to the toolbox. Frontiers in Marine Science. The data provided here contains- raw paired end sequencing data in fastq format for each sample- R markdown code to process the raw data in order to identify unique sequences, and which sequences are contained in which samples- a tag file that identifies which multiplex-identifier (MID) tags were used in forward and reverse primers for each sample- a zOTU table that identifies which sequences were detected in each sample, and what taxonomy was associated with each sequence.- video footage from each CTD station, both from the surface and the seafloor