Protea repens whole transcriptome count data for control and drought treatment for 8 populations, climatic data for the 8 populations and phenotypic data collected, and data used for linear mixed models for climate gene expression/trait correlation testing

Long term environmental variation often drives local adaptation and leads to trait differentiation across populations. Additionally, when traits change in an environment-dependent way through phenotypic plasticity, the genetic variation underlying plasticity will also be under selection. These processes could create a landscape of differentiation across populations in traits and their plasticity. Here, we performed a dry-down experiment under controlled conditions to measure responses in seedlings of a shrub species from the Cape Floristic Region, the common sugarbush (Protea repens). We measured morphological and physiological traits, and sequenced whole transcriptomes of leaf tissues from 8 populations that represent both the climatic and the geographic distribution of this species. We found that there is substantial variation in how populations respond to drought, but we also observed common patterns such as reduced leaf size and leaf thickness, and upregulation of stress- and down-r...长期环境变异通常会推动本地适应，并引发不同种群间的性状分化。此外，当性状通过表型可塑性（phenotypic plasticity）以环境依赖的方式发生改变时，支撑该可塑性的遗传变异也会受到选择作用。上述过程或可塑造出种群间性状及其可塑性的分化格局。本研究在可控条件下开展了逐步干旱实验，以测定来自开普植物区（Cape Floristic Region）的灌木物种普通糖布什（Protea repens）的幼苗响应。我们针对覆盖该物种气候与地理分布范围的8个种群的叶片组织，测定了形态与生理性状，并完成了全转录组（whole transcriptome）测序。研究发现，不同种群对干旱的响应存在显著差异，但同时也观察到诸如叶片尺寸减小、叶片厚度降低，以及胁迫相关基因的上调与……