Single-cell Transcriptome of Bronchoalveolar Lavage Fluid Reveals Dynamic Change of Macrophages During SARS-CoV-2 Infection in Ferrets

Single-cell transcriptome of bronchoalveolar lavage fluid cells longitudinally obtained from SARS-CoV-2-infected ferrets was analyzed. Changes in cell proportions and characteristics in uninfected control, at 2 days post-infection (dpi), and 5 dpi were noted. Macrophages were classified into the subpopulations with dynamic changes quantitatively and qualitatively over time. Fundamental aspects of the immune response dynamics provoked by SARS-CoV-2 infection were highlighted in this study. Total 10 single cell RNA seq libraries (10X Chromium, v3) were sequenced. All samples are bronchoalveolar lavage fluid cells from ferret. Samples are consist with 3 control (healthy) ferrets, 3 ferrets at 2 dpi of SARS-CoV-2, and 4 ferrets of 5 dpi.