APL1B modifies APL1A or APL1C function, modulating Plasmodium infection intensity but not infection prevalence.

A.APL1B activity does not influence permissiveness of mosquitoes to P. falciparum infection, because no difference in infection prevalence is seen when APL1B is silenced alone (APL1B+GFP). When APL1B is co-silenced with APL1A, the infection prevalence is no different than after silencing of APL1A alone (APL1A+B; detailed statistical comparisons in S3 Table). B. APL1B influences infection intensity of P. falciparum, a phenotype detectable only in an APL1A-depleted background (APL1A+B). C. APL1B does not influence permissiveness for P. yoelii infection, because silencing of APL1B alone or in combination with APL1C has no effect on infection prevalence. D. APL1B influences infection intensity of P. yoelii, but the increased infection intensity caused by APL1B loss-of-function is only detectable in an APL1C-depleted background (APL1C+B as compared to APL1C+GFP). Graph labels and statistical tests as in Fig 2 legend.