Gene expression in diploid and evolved tetraploid RPE-1 and BJ-1 cells

Both diploid RPE-1 and BJ-1 cells were made tetraploid by transient treatment with the cytokinesis inhibitor DCD. Proliferating tetraploids from both BJ-1 and RPE-1 were selected and isolated. The gene expression profiles of the proliferating tetraploid cells were then compared to the diploids from which they originated. Triplicate biological samples were collected and analyed.