mRNA sequencing of PPD3 over expression in Col-0 background in Arabidopsis. mRNA sequencing of PPD3 over expression in Col-0 background in Arabidopsis

The PPD3 genomic DNA was cloned in the binary vector pCAMBIA1302C with its native promoter of about 1.5 kb and was tagged with GFP. Wild type Col-0 plants were transformed by the dipping method and screened for positive plants by hygromycin and also by PCR primers for presence of the transgene. Overall design: Two plants each for both PPD3 over expression and Col-0 wild-type control were sequenced