Gene expression profile at single cell level of CD45+ immune cells in both peripheral blood mononuclear cells (PBMCs) and allograft biopsy samples obtained from kidney transplant recipients

Transplant rejection is mediated by immune cells, especially T cells. In this study, we employed single-cell RNA sequencing (scRNA-seq) to analyze the states of these cells in response to kidney transplantation Overall design: Subjects were retrospectively selected following prospective recruitment and consent at the time of renal biopsy for clinical indications (protocol biopsy for high immune risk, “for cause” such as allograft dysfunction, and post-rejection treatment). Live CD45+ cells were sorted from PBMC or kidney biopsy samples and analyzed by scRNA-seq