Transcriptome wide mapping of Hfq mediated RNA-RNA interactions in E. coli (RIL-seq). Transcriptome wide mapping of Hfq mediated RNA-RNA interactions in E. coli (RIL-seq)
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资源简介：
Most E. coli sRNAs interact with their mRNA targets through simultaneous binding to the Hfq chaperon. In this experiment we cross-linked RNA to proteins in-vivo then did Hfq IP followed by ligation of bound RNAs and sequencing to identify sRNA-mRNA interactions. We termed the method RIL-seq for RNA Interactions by Ligation - sequencing.
创建时间：
2016-07-31
