RNA-seq of the lens transition zone in control vs Pea3-depleted mouse embryonic tissue

The goal of the study is to find the transcriptional targets downstream of Pea3-mediated signaling during lens development. In order to look for the potential targets, we performed laser capture microdissection of the lens epithelial tissue at embryonic day E14.5 from control embryos as well as mutant embryos containing lens-specific deletion of Pea3 transcription factors. After tissue harvest, RNA was extracted. Conversion to cDNA as well as amplification was performed by Clontech SMART-seq v4 Ultra low input RNA kit, as well as cDNA library construction was performed using Nextera XT DNA library preparation kit by core facility at Columbia university prior to RNA sequencing. After preparation of cDNA library, each sample was sequenced using Illumina platform Overall design: For this experiment, 3 control sample and 3 mutant sample were analysed.