Single cell RNA-sequencing of Spike-ins and mESC using STRT-Seq on C1 System. Single cell RNA-sequencing of Spike-ins and mESC using STRT-Seq on C1 System

In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific methods. In this dataset, we perform STRT-seq method on Fluidigm C1 system and generate single-cell libraries using Nextera XT kit. Please note the sample-data relationship format (SDRF) file for this submission contains only a high-level representation of all sample, library and run information, and not per cell. For meta-data at the level of individual cells, please refer to the supplementary file called "single_cells_list.txt", which is included as part of this ArrayExpress submission.