Additional file 1 of Downregulation of salusins alleviates hypertrophic cardiomyopathy via attenuating oxidative stress and autophagy

Additional file 1: Figure S1. Levels of salusins after TOR2A downregulation. a Levels of salusin-α and salusin-β were reduced in the heart of rats after TOR2A knockdown. b Levels of salusin-α and salusin-β were reduced in the NRCMs after TOR2A knockdown. c Levels of salusin-α and salusin-β were reduced in the NRCFs after TOR2A knockdown. a Saline + Control shRNA and Saline + TOR2A shRNA groups (n=7), and Ang II + Control shRNA and Ang II + TOR2A shRNA groups (n = 8). b, c N = 6 in each group. Ang angiotensin, TOR2A torsin family 2 member A, NRCMs neonatal rat cardiomyocytes, NRCFs neonatal rat cardiac fibroblasts. Figure S2. Levels of body weight. There was no significant difference in the body weight among four groups. Saline + Control shRNA and Saline + TOR2A shRNA groups (n = 7), and Ang II + Control shRNA and Ang II + TOR2A shRNA groups (n = 8). Figure S3. Effects of TOR2A downregulation on cardiac function. There was no significant difference in the EF, FS LVVs, LVVd, LVIDs and LVIDd among four groups. Saline + Control shRNA and Saline + TOR2A shRNA groups (n=7), and Ang II + Control shRNA and Ang II + TOR2A shRNA groups (n=8). Figure S4. Levels of cell viability. a There were no significant differences in the NRCMs survival rate treating with Ang II and TOR2A knockdown. b There were no significant differences in the NRCFs survival rate treating with Ang II and TOR2A knockdown. N=6 in each group. Ang angiotensin, TOR2A torsin family 2 member A, NRCMs neonatal rat cardiomyocytes, NRCFs neonatal rat cardiac fibroblasts. Table S1. List of utilized primers for quantitative real time-PCR (qRT-PCR).