single cell RNA Sequencing of hippocampi of mice subjected or not to the learned helplessness paradigm

The goal of the study was to identify the hippocampal microenvironment asscoaited with stress, in particular stress-induced learned helplessness. By comparing chronic and acute induction of learned helplessness, we identified cell populations and genes associated with resilience. Overall design: Mice were subjected or not to the learned helplessness paradigms (either acute: mice received footshocks and were tested the next day, or chronic: mice received the acute paradigm and are retested for 3 weeks, weekly), and hippocampal CD45+ live dead - cells were sorted, and 10X Genomics single cell RNA Sequencing was performed.