Quality Controls.

aOne region of an 8 gasket PTP was used for a positive control (C). Negatives, positives, and pilot samples (representing a subset of three subjects) were sequenced in one region on the same run, and pilot l (A) and negative control (B) sequences partitioned by MID.
bQIIME restrictions: Minimum length  =  100 (after trimming forward primer and MID); maximum “N” = 1, maximum homocopolymer  = 10; maximum forward primer mismatch  = 2; maximum barcode mismatch  = 2.
cGenus assignments (sequence counts): Unclassified fungi (6 sequences); Saccharomyces (3); Tumularia (3); Malassezia (2); Rhodotorula (2); Candida (1); Ceratobasidium (1); Galerina (1). One of the genera assignments (Ceratobasidium) was at a very weak E-value (3.9); all others were at very strong E-values (−85 to −177).
dIncludes Candida. All non-Candida genera are constituted by singleton sequences; 4 (Scutellospora,Tomentella, Saccharomyces, Cryptococcus) have very weak E-values (0.11–4.8); 1 (Fusarium) has a strong E-value (−119).