ITS sequencing of larval Asian longhorned beetle guts collected from insects feeding on Acer and Populus spp.

Larval Asian longhorned beetles were reared in Acer spp., Populus nigra, and Populus tomentosa to determine the impacts of feeding in multiple tree species on the fungal gut community. After the insects fed in their hosts for two weeks, guts were dissected, DNA was extracted using the MoBio Power Soil DNA isolation kit, and ITS amplicons were generated using the ITS5 and ITS4 primers.