Reproductive resilience: Pathways to gametogenic success in Montipora capitata after bleaching

Thermal bleaching, or the loss of symbiotic algae that provide most energetic resources for the coral host, is an increasing threat to reefs worldwide and is projected to worsen with climate change. While this threat to coral persistence is well recognized, the impact on the essential process of reproduction in corals that survive bleaching is not well resolved despite being central to coral resilience. Montipora capitata can survive thermal bleaching while completing a full gametogenic cycle, offering an ideal system to study gametogenic resilience and associated physiological tradeoffs. We experimentally bleached fragments of M. capitata colonies and followed their gametogenic cycle and physiological responses for 10 months at six time points. All bleached colonies produced gametes at the same time as controls, suggesting that reproductive processes were energetically prioritized. However, proteomic analysis across the gametogenic cycle revealed tradeoffs and delays in activating key ..., Seventy-four M. capitata coral (approximately 30 cm in diameter) were collected from patch reefs located in KāneÊ»ohe Bay, OÊ»ahu, HawaiÊ»i around the HawaiÊ»i Institute of Marine Biology (HIMB, 21.428°N, 157.792°W) in August 2017. Each coral was divided in half, with one half assigned to the control group and the other to the thermal bleaching group. Samples were collected pre-bleaching and at 5 time points post-bleaching and through spawning. Histology was perfomed on the last two time points during the spawning season (June and July). Using histology, data were collected on oocyte and spermatocyte stage, oocyte Feret diameter, and fecundity. Chlorophyll, symbiont counts, symbiont clade (using qPCR), and total lipids were also measured. Protein was extracted from each coral sample, digested with trypsin, and analyzed on a Q-Exactive-HF mass spectrometer using data dependent acquisition. Raw mass spectra were searched against a species-specific predicted proteome using Comet and Peptide an..., , # Data from: Reproductive resilience: Pathways to gametogenic success in Montipora capitata after bleaching [https://doi.org/10.5061/dryad.x3ffbg7t8](https://doi.org/10.5061/dryad.x3ffbg7t8) This dataset contains data files and R code necessary to reproduce the figures and analysis presented in the accompanying manuscript published in Scientific Reports, \"Reproductive resilience: Pathways to gametogenic success in *Montipora capitata* after bleaching\". This includes figures summarizing the histological data and analysis with linear models; figures and linear models of total lipid, chlorophyll, and symbiont data; PLS-DA (partial least squares discriminant analysis) of the proteomics data; hierarchical cluster analysis of the proteomics data; and symbiont qPCR analysis. ## Description of the data and file structure **Histology:** montipora time series oocytes.csv * *Sample:* Unique coral sample identifier * *Treatment*: whether or not the coral fragment underwent experimental bleac...热白化（thermal bleaching），即丧失为珊瑚宿主提供绝大多数能量资源的共生藻类，是全球珊瑚礁面临的日益严峻的威胁，且预计将随气候变化进一步恶化。尽管人们已充分认识到这一威胁对珊瑚存续的影响，但对于在白化后存活的珊瑚而言，其核心恢复力来源——繁殖这一关键生理过程所受的影响仍未明确。尖孔珊瑚（Montipora capitata）可在经历热白化后存活并完成完整的配子发生周期，为研究配子发生恢复力及相关生理权衡提供了理想的实验体系。我们对尖孔珊瑚的菌落片段开展了实验性白化处理，并在10个月内的6个时间点跟踪其配子发生周期与生理响应。所有白化菌落均与对照组同期产生配子，这表明繁殖过程被优先分配能量资源。然而，贯穿配子发生周期的蛋白质组学分析揭示了关键激活过程的权衡与延迟…… 2017年8月，我们于夏威夷瓦胡岛卡内奥赫湾（Kāneʻohe Bay）的斑礁中采集了74株尖孔珊瑚（直径约30 cm），采集地点位于夏威夷海洋生物学研究所（Hawaiʻi Institute of Marine Biology, HIMB, 21.428°N, 157.792°W）附近。每株珊瑚均被分为两半，一半归入对照组，另一半归入热白化实验组。分别在白化前、白化后5个时间点以及产卵期采集样本。在产卵季的最后两个时间点（6月与7月）开展了组织学检测。通过组织学分析，我们收集了卵母细胞与精母细胞阶段、卵母细胞费雷特直径（Feret diameter）以及繁殖力的数据。此外还测定了叶绿素含量、共生藻计数、共生藻分支（通过定量PCR，qPCR）以及总脂质含量。从每份珊瑚样本中提取蛋白质，经胰蛋白酶消化后，采用数据依赖性采集模式在Q-Exactive-HF质谱仪上进行分析。原始质谱数据通过Comet和Peptide（原文未完整）针对物种特异性预测蛋白质组进行检索。 # 数据来自：《繁殖恢复力：白化后尖孔珊瑚（Montipora capitata）配子发生成功的通路》 https://doi.org/10.5061/dryad.x3ffbg7t8 本数据集包含复现发表于《科学报告》（Scientific Reports）的配套论文中图表与分析所需的数据文件与R代码，该论文题为《繁殖恢复力：白化后尖孔珊瑚（Montipora capitata）配子发生成功的通路》。其中涵盖组织学数据及线性模型分析的汇总图表；总脂质、叶绿素与共生藻相关数据的图表及线性模型；蛋白质组学数据的偏最小二乘判别分析（PLS-DA, partial least squares discriminant analysis）；蛋白质组学数据的层次聚类分析；以及共生藻定量PCR分析。 ## 数据与文件结构说明 **组织学（Histology）：** montipora time series oocytes.csv * *样本（Sample）*：唯一珊瑚样本标识符 * *处理组（Treatment）*：珊瑚片段是否接受实验性白化处理……（原文未完整）