Genetic differentiation in the boreal dragonfly Leucorrhinia dubia in the Palearctic region

The last glacial period had a strong influence on the population genetic structure of boreal species in southern and central Europe. In addition, recent and current human impact on the boreal environment has led to habitat loss, which also has a large influence on population genetic structure of species. Here we present the spatial genetic structure of the boreal dragonfly Leucorrhinia dubia using ddRAD sequencing. We sampled individuals from nine locations in Europe, three in Asia (Russia and Japan) and one location of L. intermedia in Japan. Results showed three distinct genetic clusters in Europe. One genetic cluster consisted of individuals sampled from the locations in the Swiss Alps, a second consisted of individuals sampled in the United Kingdom, and a third cluster consisted of individuals from the rest of the seven sampled locations in Europe covering a latitudinal gradient from the French Pyrenees to the north of Finland. There was also a week support that the French Pyrenees ...末次冰期对欧洲南部与中部的寒带（boreal）物种的种群遗传结构具有显著影响。此外，近期及当前人类对寒带环境的干扰已引发栖息地丧失，这同样会对物种的种群遗传结构产生重大影响。本研究利用ddRAD测序技术，解析了寒带蜻蜓狭腹灰蜻（Leucorrhinia dubia）的空间遗传结构：我们采集了欧洲9个采样点、亚洲3个采样点（俄罗斯与日本）的个体，以及日本地区的中间灰蜻（Leucorrhinia intermedia）1个采样点的个体。研究结果显示，欧洲地区存在3个显著的遗传聚类群：其中一个聚类群包含采自瑞士阿尔卑斯山各采样点的个体，第二个聚类群包含采自英国的个体，第三个聚类群则涵盖欧洲其余7个采样点的个体，这些采样点的分布纬度跨度从法国比利牛斯山直至芬兰北部。同时存在弱支持证据表明，法国比利牛斯山地区……