S1 File - Impact of storage conditions on the stability and biological efficacy of trans-arachidin-1 and trans-arachidin-3

Figure S1. Line graphs showing the stability trends of (a) ABTS antioxidant activity and (b) total phenolic content in peanut hairy root culture crude extracts (PCE) stored at room temperature (RT), 4 °C, and −20 °C over a three-month period. Figure S2. HPLC chromatograms of (a) the culture medium without elicitation (negative control) and (b) peanut hairy root culture crude extracts (PCE). Figure S3. Line graphs showing the stability of (a) trans-arachidin-1 (Ara-1) and (b) trans-arachidin-3 (Ara-3) contents in peanut hairy root culture crude extracts (PCE) stored at room temperature (RT), 4 °C, and −20 °C over a three-month period. Figure S4. Concentration–response curves showing percentage cell viability of SW480 colon cancer cells treated with peanut hairy root culture crude extract (PCE) at increasing concentrations following storage at room temperature (RT), 4 °C, and −20 °C at different time points: (a) 0 month (initial); (b) 1 month; (c) 2 months; and (d) 3 months. Figure S5. High-resolution mass spectrum (ESI-MS/MS) spectrum and plausible fragmentations of trans-arachidin-1. Figure S6. High-resolution mass spectrum (ESI-MS) spectrum of trans-arachidin-3 Figure S7. 1H NMR spectrum (400 MHz, acetone-d6) of trans-arachidin-1 Figure S8. 13C NMR spectrum (100 MHz, acetone-d6) of trans-arachidin-1 Figure S9. 1H NMR spectrum (400 MHz, acetone-d6) of trans-arachidin-3 Figure S10. 1H NMR spectra (400 MHz, acetone-d6) of trans-arachidin-1 (Ara-1) and trans-arachidin-3 (Ara-3). Figure S11. Line graphs showing the stability of peanut hairy root culture extracts stored at −20 °C over a six-month period: (a) crude extract (PCE), (b) partially purified fraction enriched in trans-arachidin-1 (Ara-1), and (c) partially purified fraction enriched in trans-arachidin-3 (Ara-3). Figure S12. Cytotoxic activity of PCE derived from peanut hairy root culture media and partially purified fractions of trans-arachidin-1 (Ara-1) and trans-arachidin-3 (Ara-3) against KKU-100 cholangiocarcinoma cells following storage at –20 °C. (a–c) show concentration–response effects expressed as percentage cell viability at the initial time point (0 month), while (d–f) show the corresponding effects after 6 months of storage: (a, d) PCE; (b, e) partially purified fractions of Ara-1; and (c, f) partially purified fractions of Ara-3. Table S1. Replicate data (N₁–N₃) for ABTS antioxidant activity and total phenolic content of peanut hairy root culture crude extracts (PCE) stored at room temperature (RT), 4 °C, and −20 °C over a three-month period. Table S2. Replicate data (N₁–N₃) for of trans-arachidin-1 (Ara-1) and trans-arachidin-3 (Ara-3) contents in peanut hairy root culture crude extracts (PCE) stored at room temperature (RT), 4 °C, and −20 °C over a three-month period. Table S3. Replicate data (N₁–N₃) for IC₅₀ values of peanut hairy root culture crude extracts (PCE) against SW480 colon cancer cells following storage at room temperature (RT), 4 °C, and −20 °C over a three-month period. Table S4. Replicate data (N₁–N₃) for ABTS antioxidant activity of peanut hairy root culture crude extracts (PCE), and partially purified fractions of trans-arachidin-1 (Ara-1) and trans-arachidin-3 (Ara-3) stored at −20 °C, evaluated at 0 and 6 months. Table S5. Replicate data (N₁–N₃) for IC₅₀ values of peanut hairy root culture crude extracts (PCE) and partially purified fractions of trans-arachidin-1 (Ara-1) and trans-arachidin-3 (Ara-3) against KKU-100 cholangiocarcinoma cells following storage at −20 °C, evaluated at 0 and 6 months. (RAR)