Flies infected with new over-proliferative Wolbachia variants did not inherit mutated mitochondria.

Illumina reads on flies infected with different Wolbachia variants were mapped to the mitochondria of D. melanogaster Release 6 genome sequence (KJ947872.2:1–14,000). A summary of the mapping is given in S2 Table. The mitogenome of flies infected with wMelCS_b, wMelOctoless and wMelPop2 was identical. We found an SNP unique to flies infected with wMelCS-like variants (G→A on position 10,793) but absent in flies infected with wMelPop. We confirmed this SNP using Sanger sequencing. (XLS)