Epigenetic Switch Reshapes Epithelial Stem/Progenitor Cells Signatures and Contributes to Inflammatory Programming in Hidradenitis Suppurativa [CUT&RUN]

1) By using dual-omics profiling for both chromatin accessibility and gene expression as well as CUT&RUN sequencing for chromatin modification states, we identified that numerous disease-associated inflammatory enhancers were epigenetically activated and co-exist with the transcriptional states of their target genes in basal progenitor cells. 2) By using CUT&RUN sequencing , we identified numerous S100A8 binding DNA sequences in HS epidermal cells. 1) The Institutional Review Board of the University of Alabama at Birmingham approved the protocol (IRB-300005214) for obtaining surgically discarded skin tissues from heathy and HS (Hurley II/III stage) subjects. Surgical excisions from 4 patients with HS (axillary or groin lesions) and 2 healthy controls (discarded tissue from breast or abdominoplasty reduction surgery) were collected. 2) The Institutional Review Board of the University of Alabama at Birmingham approved the protocol (IRB-300005214) for obtaining surgically discarded skin tissues from heathy and HS (Hurley II/III stage) subjects. Surgical excisions from 3 patients with HS (axillary or groin lesions) and 2 healthy controls (discarded tissue from breast or abdominoplasty reduction surgery) were collected.