List of oligonucleotides.
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Primers 1–5 were used to isolate and modify the cDNA encoding E. histolytica DMC1 and RAD51. H3, OL83-1, and OL90 were 32P-radiolabeled using [32P-γ]-ATP and T4-PNK. 32P-H3 and 32P-OL83-1 were annealed with H3c and OL83-2 oligonucleotides, respectively, to form double-stranded DNA substrates. 32P-OL90 was used in the D-loop and nuclease protection assay.
List of oligonucleotides.
创建时间：
2015-09-30
