Construction of Mate Pair Full-length cDNAs Libraries and Characterization of Transcriptional Start Sites and Termination Sites. Homo sapiens

To identify and characterize transcript structures ranging from the transcriptional start sites (TSSs) to the termination sites (TTSs), we constructed and analyzed TSS/TTS mate pair full-length cDNA libraries from 18 kinds of human cell types. Furthermore, we applied the similar strategy to construct cDNA libraries in which 3’-end mate pairs distribute randomly over the transcripts. We show the TSS/Random libraries were useful to assembly internal structure of the previously uncharacterized alternative promoter products as well as intergenic transcripts. This novel method to analyze full-length cDNAs should be useful to complement currently RNA Seq analyses and genome annotations.