Additional file 2 of Bisphenol A and bisphenol S both disrupt ovine granulosa cell steroidogenesis but through different molecular pathways

Additional file 2: Supplementary Table 1. Comparisons of the effect on progesterone (A) and oestradiol (B) secretion between conditions, including the p-values for all the multiple comparisons of the post-hoc test that followed the ANOVA. * indicates a significant difference and # indicates a tendency. Supplementary Table 2. Total number of reads for each sample. Six biological replicates of ovine granulosa cells were sequences per experimental conditions (Control, 50 µM BPA, 50 µM BPS, 10 µM G1), after 1h of treatment. This analysis generated an average of 23.7 ± 0.5 million reads per sample, and 73.7 ± 0.2 % of the reads were uniquely aligned with the sheep reference genome (Oar_rambouillet_v1.0, chromosomes). Supplementary Table 3. Differentially expressed genes (adjusted p-value ≤ 0.05) between the four comparisons: control versus bisphenol A (BPA), control versus bisphenol S (BPS), BPA versus BPS and G1 versus control. Six biological replicates of ovine granulosa cells were sequenced per experimental conditions (Control, 50 µM BPA, 50 µM BPS, 10 µM G1), after 1h of treatment. Supplementary Table 4. Hierarchical list of differentially expressed genes in the heatmap presented in Figure 2B. Supplementary Table 5. Functional analysis of ovine granulosa cells differentially expressed genes. Functional clusters using hierarchical clustering based on Wang’s semantic similarity between GO terms respecting GO and Ward’s criterion were performed using the ViSEAGO package. Supplementary Table 6. The main biological processes of differentially expressed genes (DEG) after treatment with bisphenol A (BPA) or bisphenol S (BPS). After 1 hour of treatment, in the presence or absence (control) of BPA or BPS at 50 μM, GC (6 replicates / condition) were an RNA-seq analysis made it possible to obtain a list of differential expression genes. Biological processes from gene ontology (GO; p ≤ 0.01) terms was obtained using ViSEAGO. The table shows the 20 GO, corresponding to biological functions, the most disturbed by BPA or BPS. The logarithmic threshold p-value was set at 2, corresponding to a p-value of 0.01. Supplementary Table 7. A Comparison of gene expression at a given culture time between treatments. Gene expression is presented as mean ± SEM and normalized to the control at 12 h (different letters indicate a significant difference between treatments).