Raw data of Western blot for Figure.3h for manuscript CELL-HOST-MICROBE-D-18-00436R1

As shown in Fig. 3h, the C7a/t virus contained two C variants: HA-C (~12.5 kDa) and C7 (~10.5 kDa) proteins with an estimated molecular ratio of 1:30, whereas the WT ZIKV had only full-length C protein (~11.4 kDa). As expected, both C7a/t and WT viruses contained mature M and E proteins. As controls, culture medium from replicon-transfected cells contained only NS1 protein, with no detectable structural proteins (C, M, or E). The data suggest that C7a/t ZIKV contains both HA-C and C7 proteins. Western blot analysis of C7a/t virus. Supernatants from replicon RNA-transfected BHK-HA-C cells (day 4 p.t.), WT RNA-transfected Vero cells (day 4 p.t.), and C7a RNA-transfected BHK-HA-C cells (day 4 p.t.) were analyzed for viral proteins (HA-C, C, M, E, and NS1) using Western blotting.如图3h所示，C7a/t病毒包含两种C蛋白变异体：HA-C（约12.5 kDa）与C7（约10.5 kDa），二者估算分子比例为1:30；而野生型寨卡病毒（WT ZIKV）仅含有全长C蛋白（约11.4 kDa）。如实验预期，C7a/t与野生型寨卡病毒均包含成熟的M蛋白与E蛋白。作为对照，复制子转染细胞的培养液中仅可检测到NS1蛋白，未检出任何结构蛋白（C、M或E）。上述数据表明，C7a/t寨卡病毒同时含有HA-C与C7两种蛋白。 C7a/t病毒的蛋白质印迹（Western blot）分析。本实验对转染后第4天的复制子RNA转染BHK-HA-C细胞、转染后第4天的野生型RNA转染Vero细胞，以及转染后第4天的C7a RNA转染BHK-HA-C细胞的上清液进行检测，通过蛋白质印迹法分析其中的病毒蛋白（HA-C、C、M、E及NS1）。