Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

We used ATAC-seq to evaluate the global programme of chromtain accessibility after Dox inducible Yamanaka factors ectopic expression during this biological process. To investigate the chromatin accessibility changes for the elimination of leukemia cells after Dox inducible Yamanaka factors (Oct4,Sox2,Klf4,c-Myc) expression, we sorted GFP+ MLL-AF9 acute myeloid leukemia cells (MLL-AF9) from leukemic mice and normal c-kit+ cells (WT) from 4F transgenic mice. Leukemia cells and ckit+ cells were sorted from distinct groups at different time points