MicroRNAs as a therapeutic target in IgA nephropathy in Indian population

Immunoglobulin A Nephropathy (IgAN) is the most occurring glomerular disease with rapid development to End Stage Renal Disease requiring renal replacement therapy. Genome-wide studies suggest geographical variations in genetic susceptibility to IgAN and disease progression have been shown to play a vital role in physio pathological state. In this study, we have identified new microRNAs allied with IgAN in Indian population. The miRNA was isolated from kidney biopsies of IgAN patients and healthy control (healthy tissue from patients with renal cell carcinoma). Small RNA library preparation was performed, followed by Next Generation Sequencing on the ION S5 Torrent at 1x50 base pairs. Later the miRNA expression analyses were carried and obtained high-quality reads from BAM file. The sequence of miRNA reads was mapped to reference Human genome (hg38). The Partek Flow Genomic Analysis Software was used to compare the miRNAs expression between healthy participants and patients with IgAN.

免疫球蛋白A肾病（IgAN）是最常见的肾小球疾病，其发展迅速，可导致终末期肾病，需进行肾脏替代治疗。全基因组研究表明，在IgAN的遗传易感性和疾病进展方面存在地域差异，这些差异在病理生理状态下发挥着至关重要的作用。在本研究中，我们已在印度人群中确定了与IgAN相关的新型微RNA。该miRNA从IgAN患者的肾脏活检样本和健康对照者（来自患有肾细胞癌的患者健康组织）中分离出来。随后进行了小RNA文库的制备，并在ION S5 Torrent平台上进行1x50碱基对的下一代测序。随后进行了miRNA表达分析，并从BAM文件中获得高质量的读数。miRNA读序列与参考人类基因组（hg38）进行比对。使用Partek Flow基因组分析软件比较了健康受试者与IgAN患者之间的miRNA表达。