Rapid Analyses of Proteomes and Interactomes Using an Integrated Solid-Phase Extraction-Liquid Chromatography-MS/MS System.

Data from ProteomeXchange, PXD ID: PXD001111. Experiment: NYLON_Hela, file: OR7_20130627_NB_Nylon_Hela_1ug_top20_1.mzml. Published as part of J Proteome Res. 2014 Dec 8 . From the Abstract: {{i}} Here, we explore applications of a LC system using disposable solid-phase extraction (SPE) cartridges and very short LC-MS/MS gradients that allows for rapid analyses in less than 10 min analysis time. The setup consists of an autosampler harboring two sets of 96 STAGE tips that function as precolumns and a short RP analytical column running 6.5 min gradients. This system combines efficiently with several proteomics workflows such as offline prefractionation methods, including 1D gel electrophoresis and strong-cation exchange chromatography ... {{/i}}