CRY1-ChIP-seq in MEFs, PreiPSCs, iPSCs, and ESCs

Genome-wide occupancy of the circadian clock Cryptochrome1 (CRY1) was compared between somatic differentiated mouse embryonic fibroblasts (MEFs) and pluripotent stem cells including precursor-induced pluripotent stem cells (iPSCs), iPSCs, and embryonic stem cells (ESCs) The library was sequenced on the NovaSeq 6000 Sequencer. Sequencing was performed using paired-end flowcell chemistry and 100 cycles with additional cycles for the index reads. The NovaSeq 6000 real-time analysis software (RTA 3.4.4) converted the images into intensities and base calls. Post-processing of the run to generate the FASTQ files was performed at the Institute for Genomics and Bioinformatics (IGB), UCI.