Gene expression profile at single cell level of hematopoietic stem and progenitor cells (HSPCs) from mouse bone marrow cultured in M3434 StemCell methylcellulose media. Gene expression profile at single cell level of hematopoietic stem and progenitor cells (HSPCs) from mouse bone marrow cultured in M3434 StemCell methylcellulose media

Hematopoiesis generates a variety of cell types after M3434 culture. We treated HSPCs with small-molecule inhibitors of Acly (SB204990), Gls (CB-839), or Vehicle (DMSO) and compared them to freshly isolated HSPCs by single-cell RNA-seq and single-cell ATAC-seq to examine the diverse cell types emerging from these conditions. Overall design: HSPCs were isolated by Lineage-depletion and c-Kit enrichment by MACS cell sorting and either cultured for 2 weeks in M3434 or isolated fresh from BL/6 mice and analyzed using scRNAseq or scATAC-seq.