Mettl14 deficiency induces infertility due to implantation failure in mice. Mus musculus

Precise control of endometrial receptivity is crucial for successful embryo implantation, which is strictly regulated by ovarian steroid hormones estrogen and progesterone. Despite our better understanding of the genetic regulation downstream of hormones, we do not know much about the epigenetic regulation that occurs during early pregnancy. To investigate the role of RNA modification called N6-methyladenosine (m6A) in embryo implantation, we generated mice with conditional deletion of Mettl14, core component of m6A writer complex, in the uterus. These mice were infertile due to implantation failure. To understand the molecular mechanisms of the infertile phenotype in this mouse, we performed RNA-seq and methylated RNA immunoprecipitation sequencing (MeRIP-seq) on the uterus at the time of implantation.