Circulating exosomal microRNAs contribute to the antimigraine effects of acupuncture

We conducted this study to determine whether exosome regulation underlies the antimigraine mechanisms of acupuncture. By comparing serum samples from patients with migraine and healthy controls using high-throughput small RNA sequencing technology , we identified 705 exosomal microRNAs that are differentially expressed in patients with migraine, and this set of 705 microRNAs included five that are particularly well characterised (hsa-miR-369-5p, hsa-miR-1268b, hsa-miR-145-5p, hsa-miR-222-5p, and hsa-miR-4488). By comparing serum samples collected from patients with migraine before and after acupuncture treatment, we showed that acupuncture normalised the expression levels of those five well-characterised exosomal microRNAs. Overall design: We compared differential expressed serum-derived exosomal miRNAs between the migraine patients group and healthy controls. And we compared differential expressed serum-derived exosomal miRNAs in migraine patients before and after acupuncture