Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols. Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols

We sequenced mRNA according to several library prep protocols with known mixtures of two species of Drosophila in order to establish linear response in each protocol. Overall design: For each library prep protocol, mixtures with 0%, 5%, 10%, and 20% D. virilis total RNA was prepared, then libraries prepared according to instructions.