The Role of Histone H3 Lysine 36 Methylation in Reprogramming of fibroblasts and on Induced Pluripotent stem Cell Generation

SETD2 downregulation via two independent shRNA infection significantly modulated expression profile of dH1f cells in preOSKM and postOSKM samples such that 186/121 genes were down and 135/66 were up-regulated in preOSKM, and 467/544 were down and 313/586 were up-regulated in postOSKM cells significantly (padj<0.001 and log2 fold change>0.5). Examination of SETD2 downregulation effect on gene expression profile of dH1f cells in pre- and 6 days postOSKM samples. 2 sets of samples were prepared for shSETD2-1/shSETD2-3 preOSKM and 3 sets of samples were prepared for shCntrl preOSKM, shSETD2-1/ shSETD2-3 and shCntrl postOSKM conditions.