Elevated EGR1 Binding at Enhancers in Excitatory Neurons Correlates with Neuronal Subtype-Specific Epigenetic Regulation

In this study, we generated epigenetic maps profiles to perform comparative histone modification analysis between excitatory and inhibitory neurons. We found that neuronal cell-type specific histone modifications are enriched in super enhancer regions containing abundant EGR1 motifs. Further CUT&RUN data validated that more EGR1 binding sites can be detected in excitatory neurons and primarily located in enhancers. RNA-seq data generated from FACS-sorted excitatory and inhibitory nuclei provided additional evidence supporting the neuronal subtype-specific regulatory function of EGR1. Overall design: RNA-seq was performed for the FACS-sorted excitatory and inhibitory nuclei from adult mouse brain.