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TABLE S 1. PCR protocols for 16 S, 28 S in A new genus of the tribe Blondeliini (Diptera: Tachinidae: Exoristinae) from the Oriental Region with morphological and molecular evidence

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Zenodo2024-08-19 更新2026-04-07 收录
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TABLE S1. PCR protocols for 16S, 28S and white amplification. DNA polymerasePCR protocols for 16S and 28S amplificationExTaq95℃ for 60 s; 40 cycles of 95℃ for 45 s, 50℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 sGflex95℃ for 180 s; 40 cycles of 98℃ for 10 s, 50℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 sNumber of rounds and DNA polymerasePCR protocols for white amplificationFirst-round for ExTaq95℃ for 60 s; 40 cycles of 95℃ for 45 s, 45℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 sFirst-round for Gflex95℃ for 180 s; 40 cycles of 98℃ for 10 s, 45℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 sSecond-round for ExTaq95℃ for 60 s; 40 cycles of 95℃ for 45 s, 55℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 sSecond-round for Gflex95℃ for 180 s; 40 cycles of 98℃ for 10 s, 55℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s

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2024-08-19
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