Grey Mackerel (Scomberomorus semifasciatus) microsatellite genotypes (nine loci) representing twelve regions along Australia's northern coastline from the eastern coast of Queensland to the western coast of Western Australia.
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资源简介:
Scomberomorus semifasciatus is an Australian endemic found in tropical, coastal waters from eastern to western Australia. Commercial and recreational exploitation is common and regulated by state-based authorities. This study used mitochondrial DNA sequence and microsatellite markers to elucidate the population structure of Scomberomorus semifasciatus collected from twelve, equidistant sampling locations. Samples (n=544) were genotyped with nine microsatellite loci, and 353 were sequenced for d-loop (384 bp) and ATP (800bp) mitochondrial DNA gene regions. Combined interpretation of microsatellite and mtDNA data identified four genetic stocks of S. semifasciatus: Western Australia, northwest coast of the Northern Territory, Gulf of Carpentaria and the east coast of Queensland. Connectivity among stocks across northern Australia from the Northern Territory to the east coast of Queensland was high, but in contrast, there was a clear genetic break between populations in Western Australia compared to the rest of northern Australia. This indicates a restriction to gene flow possibly associated with suboptimal habitat along the Kimberley coast (northwestern Australia). The appropriate scale of management for this species corresponds to the jurisdictions of the three Australian states, except that the Gulf of Carpentaria stock should be co-managed by authorities in Queensland and Northern Territory.
带纹马鲛(Scomberomorus semifasciatus)为澳大利亚特有物种,栖息于澳大利亚东部至西部的热带近岸海域。该物种的商业捕捞与休闲垂钓活动较为普遍,相关活动受各州主管部门监管。
本研究采用线粒体DNA(mitochondrial DNA, mtDNA)序列与微卫星标记(microsatellite markers),对采自12个等距采样点的带纹马鲛样本进行种群结构解析。共获取544尾样本,对其9个微卫星位点完成基因分型;其中353尾样本的线粒体DNA D-loop区(384 bp)与ATP基因区域(800 bp)得以测序。
结合微卫星标记与线粒体DNA数据的联合分析,共鉴定出带纹马鲛的4个遗传种群:西澳大利亚种群、北领地西北海岸种群、卡奔塔利亚湾种群以及昆士兰州东海岸种群。从北领地至昆士兰州东海岸的澳大利亚北部海域各遗传种群间连通性较高;与之形成鲜明对比的是,西澳大利亚种群与澳大利亚北部其余种群之间存在显著的遗传分化断裂。
该结果表明种群间基因流(gene flow)存在限制,其可能与澳大利亚西北部金伯利沿岸的适宜性欠佳的栖息环境相关。该物种的适宜管理尺度应匹配澳大利亚三个州的管辖范围,仅卡奔塔利亚湾种群需由昆士兰州与北领地的主管部门共同管理。
提供机构:
Queensland Department of Primary Industries



