Transcriptome profiling of oxidase knockout mice response to gingival inflammation using RNA-seq

Genetic defects in neutrophil function or trafficking are highly associated with oral inflammation and periodontitis. The leukocyte NADPH oxidase enzymes complex is a multi-subunit enzyme that has been described to play important immunomodulatory roles in limiting the inflammatory responses of neutrophils and macrophages. Here, we determined the impact of loss of NADPH oxidase dependent reactive oxygen species (ROS) generation in gingival inflammatory responses using a model of ligature induced murine periodontitis. Total RNA was isolated from inflamed gingival tissues (tissue surrounding the second maxillary ligated molar) as well as gingival tissues of naïve mice. 3 age and sex matched mice were used per group. Libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit along with TruSeq RNA CD Index Plate from Illumina. Sequencing runs were performed on the Illumina NextSeq 500 using the NextSeq 500/550 75 cycle High Output Kit v2.5.