Wound image and transcriptome datasets of swine acute wounds

Wound healing progresses through overlapping phases: hemostasis, inflammation, proliferation, and remodeling. Continuous characterization of these transitions remains limited. Here, we employed a swine excisional wound model to monitor cellular dynamics across the healing timeline. Both non-invasive imaging and wound biopsy samples from the wound edge and center were acquired. Wound photographs were analyzed using advanced artificial intelligence methods. Wound biopsy samples were subject to RNA sequencing to generate gene expression profiles for the course of healing. By combining the image and the gene expression analyses, we were able to create the comprehensive data for wound healing, which can serve as ground truth for building wound diagnostic and treatment algorithms.
, Six domestic pigs (Yorkshire-mix breed, females, 45-50 Kg) were utilized and divided to 2 groups (3 animals/group) for wound biopsy collection in a rotation order (group 1 biopsy on post-op day 1, 3, 5, 7, 11, 15, and 21/endpoint; group 2 biopsy on day 2, 4, 6, 9, 13, 16, and 19/endpoint).  Twelve full-thickness, excisional wounds at 2cm in diameter were created bilaterally on each side of the dorsum of each animal after shaving, depilation, and skin preparation.  Wound images (day 0-21) were captured with a DSLR camera at a set distance of 1 foot above the wounds, and a photo scale (Medline NE1 Wound Assessment Tool) was used for calibration in each image.  To determine the wound healing timeline, open wound areas were analyzed by manual tracking in ImageJ or automatically cropped and analyzed by algorithms.
RNA samples (a total of 150 samples of 72 paired samples from the wound edge and center, and 6 healthy skin samples as controls) were extracted from the wound biopsi..., , # Wound image and transcriptome datasets of swine acute wounds

Dataset DOI: [10.5061/dryad.0rxwdbsbr](10.5061/dryad.0rxwdbsbr)

**Description of the data and file structure**

The dataset contains observations from an acute wound healing experiment in pigs. Wound photographs and RNAseq data were collected at multiple time points from day 0 to day 21 post-wound onset. Tissue samples for RNAseq were taken from both wound edges and wound centers.

Experiment description:

Six domestic pigs (Yorkshire-mix breed, females, 45-50 Kg, pig ID: 1323, 1324, 1325, 1326, 1327, and 1328) were utilized. Twelve full-thickness, circular excision wounds (2 cm in diameter, wound ID: A, B, C, D, E, F, G, H, I, J, K, and L) were created in each animal. Baseline (Day 0) wound images and the excised skin tissue were collected.

During the post-operative period, wound images and biopsy samples from the wound edge and wound center were collected on post-operative days 1, 2, 3, 4, 5, 6, 7, 9, 11, 13, 15, 16, 19...,