A multiscale approach to decipher molecular mechanisms implicated in the intergenerational effect of ibuprofen on the mosquito Aedes aegypti. Molecular mechanisms implicated in the intergenerational effect of ibuprofen on the mosquito Aedes aegypti : focus on exposed larvae

A laboratory strain of Ae. aegypti (Liverpool strain) was exposed to ibuprofen (1µg/L)from larvae hatching to imagoes emergence. To understand the mechanistic processes underlying the direct impacts of ibuprofen on exposed larvae, we used RNA-seq based transcriptomic approach. Each condition (exposed or not to ibuprofen) was analyzed in triplicate, each RNA sample being extracted from L4 stage larvae. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads.