Comparison of NK cells and ILC1 in lean versus obese mouse livers by RNASeq

Mice were weaned onto standard RM1 diet or onto a highly palatable obesogenic diet (824018 – ‘45% AFE fat) supplemented with condensed milk. After 12 weeks, mice were killed, and liver NK cells (Lin- NK1.1+ CD49a- CD49b+) or ILC1 (Lin- NK1.1+ CD49a+ CD49b-) were sorted. Total RNA was extracted from sorted cells, cDNA generated and RNASeq performed. Cells from the livers or 3 lean and 3 obese mice were sorted, with liver NK cells and ILC1 isolated from each mouse, for a total of 12 samples