Guts from worker honey bees targeted loci. insect gut metagenome

Newly emerged workers were placed into experimental treatments (nurses present vs. not present, artificial vs. natural food) and were aged for 3 days or 7 days. The guts of these workers (not including the crop) was then dissected out, DNA was extracted, and the 16S rRNA gene was amplified from the microbial population in each sample.