Purification step, yield, and flagellin bioactivitya of candidate vaccines.
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aflagellin bioactivity was assessed by measuring secreted IL-8 with a sandwich ELISA (BD Biosciences) as previously described [9]. HEK293 cells in 96-well plates (n = 9) were stimulated with 278 ng/ml of each vaccine candidate for 16–18 hours at 37°C. IL-8 concentrations in medium were calculated from an IL-8 standard curve, and expressed as means ± SDs. IL-8 levels of cell control samples were
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2015-12-02
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