Data underlying the publication: Principles for stimulating concurrent selective microbial growth and polyhydroxyalkanoate (PHA) storage responses for enhanced PHA productivity

Description:The aim of this study was to investigate whether reduced ATP demand, combined with net NADH generation in the conversion of substrates to PHA, correlates with concurrent selective growth response of the PHA-storing fraction in WAS. Furthermore, the study sought to determine if such selective growth significantly enhances PHA productivity in a direct accumulation bioprocess. Outcomes of replicate butyrate direct accumulations were studied with respect to parallel accumulations performed with acetate. Two distinct sources of municipal WAS were evaluated with replicate experiments for relative effects of the substrates on process mass and ATP balances, microbial community developments, and the PHA accumulation performance indicators.<br>This information is being made public as supplementary data for the publications and PhD thesis of Yizhou Xing, and other researchers are encouraged to utilize this data in their work.<br>The data in this dataset was collected in Wetsus, Leeuwarden, the Netherlands between May 2023 and December 2024. <br>This dataset is published under the CC BY (Attribution) license.

本研究旨在探究：底物转化生成聚羟基脂肪酸酯（Polyhydroxyalkanoates, PHA）过程中降低的三磷酸腺苷（ATP）需求与净烟酰胺腺嘌呤二核苷酸（还原态, NADH）生成，是否与剩余活性污泥（Waste Activated Sludge, WAS）中储存PHA的菌群组分的同步选择性生长响应相关。此外，本研究还试图明确此类选择性生长是否可在直接累积生物过程中显著提升PHA产率。本研究针对以乙酸盐为底物的平行累积实验，分析了重复进行的丁酸盐直接累积实验结果。通过重复实验评估了两种不同来源的市政剩余活性污泥，以对比底物对过程物料与ATP平衡、微生物群落动态及PHA累积性能指标的相对影响。<br>本数据集作为邢亦舟（Yizhou Xing）已发表论文及博士学位论文的补充数据公开，同时鼓励其他研究人员在其研究工作中使用该数据。<br>本数据集的数据采集工作于2023年5月至2024年12月间，在荷兰吕伐登（Leeuwarden）的Wetsus研究中心完成。<br>本数据集采用知识共享署名（CC BY）许可协议发布。