SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in June 2009

Flow cytometry data was collected in June2009, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), B4 (35.168S, 135.418E) and B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.本数据集的流式细胞术（Flow Cytometry）数据采集于2009年6月，采集区域为澳大利亚南部近岸海域。本研究的核心目标为：探明澳大利亚南部大陆架海域的生态系统背景信息，阐明上升流与盐度异常外流事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托恩格林号科考船（RV Ngerin）开展，属于澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目的组成部分。每趟科考航次中，研究人员均对叶绿素荧光最大值（chlorophyll fluorescence maximum, FM）层的物理、化学与生物学特性开展了调查。本次采集的流式细胞术数据涵盖超微型浮游植物、细菌与病毒三类样本。本研究共设置6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、B4（35.168°S，135.418°E）以及B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需注意的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及科考船的平均航速（约9节），可推断各站位采集的水体团并不相同。研究团队还偶尔在袋鼠岛国家参考站位（National Reference Station, NRS，35.832°S，136.447°E）以及斯宾塞湾口锚泊站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E，该区域存在盐度异常外流现象）采集补充样本。